What would be a recommended SEC MS-compatible mobile phase for use with a NIST mAb protein? - WKB202162
Article number: 202162
ENVIRONMENT
- Size exclusion chromatography (SEC)
- Mobile phase selection
- NIST mAb protein
- LC/MS
ANSWER
A mobile phase of 30% acetonitrile/0.1% TFA/0.1% formic acid has been used to separate the heavy and light chain reduced NIST mAb subunits on Waters SEC columns.
ADDITIONAL INFORMATION
For additional information, see A Platform Method for the Molecular Mass Analysis of the Light Chains and Heavy Chains of Monoclonal Antibodies using the BioAccord System
and Reliable High Resolution SEC Separations for Online Native LC-MS mAb Analysis
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