What should I do to prevent chromatographic contamination in amino acid kits like AccQ•Tag and Pico•Tag? - WKB30847
Article number: 30847
ENVIRONMENT
- AccQ•Tag
- Pico•Tag
- Amino acid analysis (AAA)
- contamination
- prevent
- avoid
- tips
ANSWER
Avoid the use of plastic containers for eluents and during derivatization of standards and samples.
Use gloves that do not contain powder.
Pyrolyze all sample tubes and reaction vials by heating them at 500 degrees Celsius for 4 hours
Run a derivatization blank as well as an underivatized blank to evaluate the background contamination that may show up in the chromatogram.
ADDITIONAL INFORMATION
The HPLC and UPLC systems should not be positioned near a fume hood or ventilation source like an air conditioning unit.
For additional information see Can I use Parafilm to re-seal my Accq-Tag Eluents for storage at 4 degrees Celsius?
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