Is there really a chromatographic difference when using methanol or acetonitrile in reverse-phase chromatography? - WKB91651
Article number: 91651
ENVIRONMENT
- Method development
- Reverse-phase chromatography
- Methanol
- Acetonitrile
ANSWER
Yes. Acetonitrile is the stronger solvent in reverse-phase separations. Compounds tend to elute faster with Acetonitrile compared with the use of methanol.
ADDITIONAL INFORMATION
Acetonitrile and methanol are used in screening method development experiments because they have different influences on reverse-phase chromatography.
For additional information, see Rapid Method Development through Proper Column Selection.
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