How can I convert a Waters Spherisorb Cyano (CN) normal phase column to run a reversed phase method? - WKB84238
Article number: 84238
OBJECTIVE or GOAL
Change the solvent inside a Waters Spherisorb CN column (which ships in 96:4 Heptane:Isopropanol) to run a reversed phase method that uses water and acetonitrile mobile phases.
ENVIRONMENT
- Waters Spherisorb
- Cyano (CN)
- Column
- Normal Phase
- Reversed Phase
- Solvent Changeover
PROCEDURE
- Flush column with 20 column volumes of 100% isopropanol.
- Flush column with 10 column volumes of 50:50 Water:Acetonitrile (do not have buffer in the water on this step, since the buffer can precipitate in the 100% IPA).
- Flush column with 10 column volumes of the initial reversed phase solvents (include buffers in this step, if buffers are used).