How do I minimize peak splitting problems when running HILIC mode chromatography? - WKB80284
Article number: 80284
SYMPTOMS
Peak Splitting
ENVIRONMENT
- column
- HILIC
- Amide
- ACQUITY
- XBridge
- UPLC
- HPLC
- polar retention
- peak split
- peak shape
- troubleshooting
CAUSE
Too much water in the sample diluent.
FIX or WORKAROUND
- Dilute the sample in 50/50 acetonitrile/water.
- Reduce the injection volume. This is determined experimentally.
ADDITIONAL INFORMATION
Water is the strong solvent in HILIC mode separation. Too much highly polar solvent can produce a strong solvent effect and influence the peak shape when injected into the weaker solvent conditions. The weaker solvent in HILIC mode separation is the acetonitrile.
For additional information, see What can cause split peaks and high pressure using a HILIC colummn?
id80284, UPBINARY