What is a good reverse-phase column choice for purification of 288 micromoles (~1.5 grams) of a 16 mer (5.3 kDa) single-stranded oligonucleotide? - WKB80168

ENVIRONMENT

• Oligonucleotide separation technology (OST)
• Oligonucleotide purification
• Optimum bed density (OBD)

ANSWER

186008964 - XBridge Oligo BEHC18 2.5µm 50x50 OBD Prep

ADDITIONAL INFORMATION

Note: Flow rates of about 120 mL/min would typically be used on a 50-mm ID prep column for this application (based on scale-up of 1.0 mL/min for a 4.6-mm ID column).

List of all Oligonucleotide Separation Technology (OST) columns:

186003952 - XBridge OST C18 2.5µm 2.1x50mm Column
186003953 - XBridge OST C18 2.5µm 4.6x50mm Column
186008212 - XBridge Oligo BEHC18 2.5µm 10x50 Prep OBD
186008962 - XBridge Oligo BEHC18 2.5µm 19x50 OBD Prep
186008963 - XBridge Oligo BEHC18 2.5µm 30x50 OBD Prep
186008964 - XBridge Oligo BEHC18 2.5µm 50x50 OBD Prep
 

Related links:

Oligonucleotide Separation Technology (OST) Columns Brochure

ACQUITY UPLC Oligonucleotide C18 Columns Care & Use Manual

XBridge Oligonucleotide C18 Columns Care & Use Manual

XBridge OST C18 Method Guidelines

ACQUITY UPLC OST C18 Method Guidelines

Oligonucleotide Applications Notebook

Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options

MassPREP OST Standard Care & Use Manual

Waters OBD Prep Column Technology: White Paper