For peptide mapping applications, which column length would provide the best separation (higher peak capacity), 150 mm or 250 mm? - WKB72460
Article number: 72460
ENVIRONMENT
- Peptide mapping
- Column length selection
ANSWER
If both columns are packed in the same packing and have the same inner diameter, a 250-mm column provides more resolution (peak capacity) for peptides, compared with a 150-mm column when run on the same system, provided the gradient is properly scaled.