What is a recommended cleaning mobile phase to remove plasma lipid buildup on a reverse-phase column? - WKB68345
Article number: 68345
ENVIRONMENT
- Reverse-phase columns
- Column care and cleaning
ANSWER
Start cleaning the column in 80:20 methanol/water. Starting in 100% solvents will precipitate peptide residues and sugars that build up on the column from the matrix. Follow the 80:20 methanol/water with recommendations for column flushes involving nonpolar solvents.
ADDITIONAL INFORMATION
For additional information, see the appropriate column care and use manual for compatible solvents and cleaning suggestions.
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