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Waters

How do I condition an Atlantis HILIC column? - WKB60072

Article number: 60072

ENVIRONMENT

  • Atlantis HILIC

ANSWER

Equilibrate in 50% acetonitrile/50% aqueous buffer (10 mM final buffer concentration) for 50 column volumes.

Prior to the first injection, equilibrate with 20 column volumes of initial mobile phase.

When running gradients, equilibrate with 10 column volumes between injections.

 

ADDITIONAL INFORMATION

Failure to appropriately equilibrate the column could result in drifting retention times.

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