How do I condition an Atlantis HILIC column? - WKB60072
Article number: 60072
ENVIRONMENT
- Atlantis HILIC
ANSWER
Equilibrate in 50% acetonitrile/50% aqueous buffer (10 mM final buffer concentration) for 50 column volumes.
Prior to the first injection, equilibrate with 20 column volumes of initial mobile phase.
When running gradients, equilibrate with 10 column volumes between injections.
ADDITIONAL INFORMATION
Failure to appropriately equilibrate the column could result in drifting retention times.
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