Can I inject samples that contain 1% SDS onto a BEH SEC column? - WKB58883
Article number: 58883
ENVIRONMENT
- Size exclusion chromatography (SEC)
- Sodium dodecyl sulfate (SDS)
- BEH SEC columns
ANSWER
Yes, but this changes column performance (see additional information).
ADDITIONAL INFORMATION
It is OK to inject samples that contain 1% SDS onto BEH SEC columns, but note the following:
1. If these columns are exposed to SDS, they will never perform the same way they did before the exposure.
2. If injecting samples with 1% SDS, you may need to include 0.1% SDS in the mobile phase.
3. The more SDS in the mobile phase, the "larger" the protein will be, so it may be necessary to go to a wider pore diameter.
ACQUITY UPLC BEH SEC Columns and Standards Care & Use Manual
XBridge Protein BEH SEC Columns & Standards Care & Use Manual
See also Can surfactants be used in the sample diluent or mobile phase when using a BEH SEC column?
id58883, eluent, MSDS