What is a good Reversed-Phase LC column choice for the analysis of lipids and/or lipidomics studies? - WKB56493

ENVIRONMENT

• ACQUITY Premier CSH C18
• ACQUITY UPLC CSH C18 columns
• ACQUITY UPLC BEH C18 columns
• ACQUITY Premier CSH Phenyl-Hexyl
• Lipidomics

ANSWER

Various columns (ACQUITY UPLC CSH C18, ACQUITY UPLC BEH C18, ACQUITY BEH HILIC, and others) have been used by Waters scientists. 

Column choice depends on the nature of the lipid classes being studied. 

ADDITIONAL INFORMATION

Visit the Waters webpage for Metabolomics & Lipidomics and click on the "Application Notes" tab.

See: Search Results | Water Lipid Reversed-Phase Applications

Some related Application Notes:

Achieving Comprehensive Lipid Profiling with a CCS, Retention Time, and MS/MS Library (uses ACQUITY Premier CSH C_18, 2.1 x 100 mm, 1.7 μm)

Rapid Analysis of Lipid Nanoparticle Components Using BioAccord LC-MS System

Lipid Analysis Using the ACQUITY RDa Detector with SmartMS Technology and UNIFI Application

Lipid Separation: UPLC System for the Separation of Complex Biological Total Lipid Extracts 

Lipid Nanoparticle Compositional Analysis Using Charged Surface Hybrid Phenyl-Hexyl Separation with Evaporative Light Scattering Detection

Journal Article:

Increasing lipidomic coverage by selecting optimal mobile-phase.pdf