What are the causes of low plate count exhibited by a column? - WKB54938

ENVIRONMENT

• Column plate count
• ACQUITY UPLC
• HPLC
• GPC

ANSWER

The column plate count is a reflection of both the column and the system working together. Low plate count can be caused by the following:

• The ferrule and compression screw not seated properly in the column hex nut
• Too much bandspread in the system for the volume of the column

ADDITIONAL INFORMATION

For additional information on system bandspread influence on column performance, see Why is my 4.6mm ID SEC and GPC column giving poor peak shape and broad peaks on an HPLC system that is normally successful with 7.8mm ID SEC and GPC columns?