What is the estimated peptide mass loading capacity for Waters Reverse-Phase Columns when performing peptide mapping? - WKB27920
Article number: 27920
ENVIRONMENT
- Peptide
- Loading Capacity
- Mass Load
- Isolation
- Purification
- Peptide Separation Technology (PST)
- Bridged Ethylene Hybrid (BEH)
- Charged Surface Hybrid (CSH)
ANSWER
See: Unique New Columns for Peptide Separations Wall Chart for estimated peptide mass loads for peptide mapping applications
ADDITIONAL INFORMATION
The best solution is to always perform an empirical loading study specific to the peptides, columns, and methods being used.
Capacity is sample and matrix specific.
- Hydrophobic peptides exhibit lower solubility in a high aqueous solution, and will have a lower loading capacity.
- Hydrophilic peptides exhibit higher solubility in a high aqueous solution, and will have a higher loading capacity.
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