Can I use a lower percentage of TMAC in the initial conditions to retain empty and full AAV capsids on a Protein-Pak Hi Res Q column? - WKB256142
Article number: 256142
ENVIRONMENT
- Adeno-associated virus (AAV) application
- Tetramethylammonium chloride (TMAC)
- Protein-Pak Hi Res Q column
ANSWER
Yes
ADDITIONAL INFORMATION
The gradient conditions in the following application should be viewed as a starting point. Some method development may be required for specific AAV serotypes.
See Figure 7b as it pertains to the salt percentage and gradient change used for the retention of AAV9: Anion-Exchange Chromatography for Determining Empty and Full Capsid contents in Adeno-Associated Virus