How do I calculate void volume and total retention volume for Styragel GPC columns? - WKB239957

ENVIRONMENT

• Column specifications
• GPC
• SEC
• Styragel
• Void volume
• Exclusion volume
• Total retention volume
• Total inclusion volume

ANSWER

The void volume in GPC and SEC is the exclusion volume of the column.

Vo = exclusion volume. Use approximately 0.4 x empty SEC/GPC column volume.

Vt = total retention volume (words used in Empower GPC software parameter) [totally included volume]. Use approximately 0.8 x empty SEC/GPC column volume.

ADDITIONAL INFORMATION

Calibration of actual molecular weight standards is required to establish the actual exclusion volume and included volume for the samples, because their size and shape are affected by the method conditions, which can include sample/standard diluent and mobile phase.

For additional information, see How do I calculate the empty column volume of a column?