What will happen to the chromatography if I use a 2.1x50mm 5µm column rather than a 2.1x100 2.5µm column? - WKB235706
Article number: 235706
ENVIRONMENT
- Chromatography column effects
ANSWER
If all other aspects of the method are held constant, the separation will be less efficient on the 5µm, 2.1x50mm column and the peaks will elute from the column in less time.
ADDITIONAL INFORMATION
Moving the method from a 2.1x100mm 2.5µm column format to a 2.1x50mm 5um column format will provide less resolution for the separation.
For additional information, see How to measure column plate count and What is meant by "column plate count"?
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