How should I clean a CORTECS C18 column if I suspect protein has precipitated? - WKB205034

ENVIRONMENT

• Column care and use
• Column cleaning
• CORTECS C18+
• DMSO
• Proteins

ANSWER

Option 1: Inject repeated aliquots of dimethyl sulfoxide (DMSO). With DMSO, the recommended cleaning injection solution should be 50:50 DMSO/H₂O in a sample vial. Then, run 100% H₂O as mobile phase and make 5 to 10 injections of the 50:50 DMSO/H₂O solution as the cleaning solution.

Option 2: Gradient of 10% to 90% B where A = 0.1% trifluoroacetic acid (TFA) in water and B = 0.15% trifluoroacetic acid (TFA) in acetonitrile. 

Option 3: Flush column with 7M guanidine hydrochloride or 7M urea. 

ADDITIONAL INFORMATION