How to install an ACQUITY OST column on the instrument - WKB201480

OBJECTIVE or GOAL

Install a newly opened ACQUITY OST column on the instrument.

ENVIRONMENT

• Oligonucleotide analysis
• OST column
• ACQUITY
• I-Class

PROCEDURE

1. Purge the system of any buffer-containing mobile phases and connect the inlet end of the column to the injector outlet.
2. Flush the column with 100% organic mobile phase (acetonitrile with TEAA or methanol for TEA-HFIP ion pairing method) by setting the pump flow rate to 0.1 mL/min and increasing the flow rate to 0.5 mL/min over five minutes.
3. When mobile phase is flowing freely from the outlet, stop the flow and attach the column outlet to the detector.
4. Gradually increase the flow rate as described in step 2.
5. Continue until a steady baseline is achieved at 260 nm. 

ADDITIONAL INFORMATION