How to install an ACQUITY OST column on the instrument - WKB201480
Article number: 201480
OBJECTIVE or GOAL
Install a newly opened ACQUITY OST column on the instrument.
ENVIRONMENT
- Oligonucleotide analysis
- OST column
- ACQUITY
- I-Class
PROCEDURE
- Purge the system of any buffer-containing mobile phases and connect the inlet end of the column to the injector outlet.
- Flush the column with 100% organic mobile phase (acetonitrile with TEAA or methanol for TEA-HFIP ion pairing method) by setting the pump flow rate to 0.1 mL/min and increasing the flow rate to 0.5 mL/min over five minutes.
- When mobile phase is flowing freely from the outlet, stop the flow and attach the column outlet to the detector.
- Gradually increase the flow rate as described in step 2.
- Continue until a steady baseline is achieved at 260 nm.
ADDITIONAL INFORMATION
id201480, eluent, Hexafluoroisopropanol