How much protein can I load onto an Ultrahydrogel column if I elute it with 50% acetonitrile? - WKB126058

ENVIRONMENT

• High Performance Liquid Chromatography (HPLC)
• UltraPerformance Liquid Chromatography (UPLC)
• Gel permeation chromatography (GPC)
• Size exclusion chromatography (SEC)
• Gel filtration chromatography (GFC)
• Ultrahydrogel
• XBridge
• Bridged ethylene hybrid (BEH)
• Protein separation
• ACQUITY

ANSWER

This would need to be determined experimentally.

Note that elution with 50% acetonitrile is not recommended because proteins will precipitate on the column in 50% acetonitrile.

ADDITIONAL INFORMATION

Ultrahydrogel columns are not designed to capture proteins via nonspecific adsorption.

Waters cannot guarantee any type of reproducible results when relying on nonspecific binding as a way to separate proteins from small molecules on Ultrahydrogel columns. Due to the charged surface of Ultrahydrogel packings, protein separation is not recommended on these columns.

Instead, diol-bonded SEC columns, such as BEH SEC columns, are recommended because proteins are not as likely to adsorb to these packings via nonspecific adsorption.

ACQUITY UPLC SEC Product Solution Brochure

XBridge Protein BEH SEC Columns Brochure