What are some potential causes of a negative dip before a peak and tailing after the peak with a GPC (gel permeation chromatography) method? - WKB126008

ENVIRONMENT

• Gel permeation chromatography (GPC)
• Negative dip
• Peak tailing
• Tubing connections

ANSWER

• The column connection to the tubing may be damaged.
• The tubing may not be seated properly inside the connection to the column.
• The ends of the tubing may be pinched, as a result of overtightening the connection.
• The tubing may be bent too much, and a kink may have formed in the tube.
• If using a new column, the column may need additional flushing.

ADDITIONAL INFORMATION

Beginner's Guide to Size-Exclusion Chromatography Primer (online guide and hardcopy publication 715004398)