Will the ACQUITY BEH Glycan column gradient cleaning recommendation of 0.1% TFA in the mobile phase affect the results of my glycan analysis? - WKB121905
Article number: 121905
ENVIRONMENT
- Glycan analysis
- Column care and use
- ACQUITY BEH Glycan
ANSWER
It should not affect the selectivity of the glycans. However, the TFA will stick around in the system and in the column for a long time and slowly clean off, with TFA adducts detected on MS.
ADDITIONAL INFORMATION
For additional information, see What is the suggested gradient conditions used to clean the ACQUITY BEH Glycan columns? and additional cleaning procedure suggestions in ACQUITY UPLC BEH Glycan, 1.7 µm Columns and Glycan Performance Test Standard.