How do I start up a new Nova-Pak C18 column? - WKB119956

OBJECTIVE or GOAL

Start up a new Nova-Pak C18 column, introducing mobile phase without damaging the column or precipitating any buffer in the mobile phase.

ENVIRONMENT

• Nova-Pak C18 column
• Column startup

PROCEDURE

1. Attach the column to the HPLC system with the outlet line flushing to a waste container.  
2. Flush the column for 10 column volumes with 50/50 acetonitrile or methanol/water.
3. Connect the column to the detector.
4. Flush the column with the mobile phase for 20 column volumes. 

ADDITIONAL INFORMATION

For additional information, see the Analytical Columns and Cartridges Care and Use Manual.