What is a recommended cleaning procedure to remove nonpolar compounds from a Resolve C18 column? - WKB112501
Article number: 112501
ENVIRONMENT
- Resolve C18 column
- Column care and cleaning
ANSWER
The column can be cleaned with the following sequence of solvents: 100% acetonitrile, 100% THF, 100% methylene chloride, 100% hexane, 100% methanol, 100% acetonitrile, mobile phase.
ADDITIONAL INFORMATION
If the mobile phase contains buffers or additives not soluble in acetonitrile, use 100% water flush prior to 100% acetonitrile.
Before any cleaning procedure is done, make sure the solvents are compatible and supported for the HPLC system.
For additional information, see How many column volumes are suggested to minimally clean a column prior to shut down?
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