What is the recommended concentration of peptide to inject on a reverse-phase column? - WKB112471
Article number: 112471
ENVIRONMENT
- Reverse-phase column
- Peptide separation technology (PST)
ANSWER
The concentration can be approximately 0.6 - 2 mg/mL. The precise concentration will be peptide-specific based on size, hydrophobicity, and chromatographic method and detection.
ADDITIONAL INFORMATION
For additional information, see Does Waters sell a peptide standard that can be used to check system and column performance? and High Mass Loading of Peptides with Hybrid Particle C18 Columns and Acetic Acid Mobile Phases.
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