What is a good UPLC column choice for analysis of disaccharide oligomers up to 10 units (20 sugar residues) in length? - WKB111550

ENVIRONMENT

• UltraPerformance Liquid Chromatography (UPLC)
• ACQUITY
• Bridged ethylene hybrid (BEH)
• Hydrophilic interaction chromatography (HILIC)

ANSWER

ACQUITY UPLC BEH Amide

ADDITIONAL INFORMATION

The ACQUITY UPLC BEH Amide column is a HILIC column.

Waters has published no application notes for the separation of medium-chain oligosaccharides. However, the BEH Amide column is a good choice for this type of separation. Use a shallow gradient of increasing polar solvent.

ACQUITY UPLC BEH Amide Column Website

ACQUITY UPLC  BEH Amide Column Brochure

ACQUITY UPLC BEH Amide Applications Notebook

Efficient Hydrophilic Interaction Chromatography (HILIC) Method Development Strategy Wall Chart

Related seminar slides:

Understanding and Creating Polar Retention Using Reversed-Phase HPLC and Hydrophilic Interaction Chromatography (HILIC)

Hydrophilic Interaction Chromatography (HILIC) For Very Polar Analytes: What Is It? Should I Be Using It? How Do I Use It?