Is it OK to inject samples that contain reducing agents of 40mM DTT, 50mM TCEP, or 2mM L-cysteine onto a BEH 300A C4 column? - WKB108998
Article number: 108998
ENVIRONMENT
- Ultra Performance Liquid Chromatography (UPLC)
- Ultra High Performance Liquid Chromatography (UHPLC)
- High Performance Liquid Chromatography (HPLC)
- Bridged ethylene hybrid (BEH)
- BEH 300A C4 column
ANSWER
Yes
ADDITIONAL INFORMATION
These concentrations of reducing agents should not harm this packing provided a gradient method starts at low organic, such as 5%.
DTT and TCEP should elute very early in the chromatogram.
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