Is it OK to inject samples that contain detergents of 1% NP40 or 0.5% SDS onto a BEH 300A C4 column? - WKB108996
Article number: 108996
ENVIRONMENT
- Ultra Performance Liquid Chromatography (UPLC)
- Ultra High Performance Liquid Chromatography (UHPLC)
- High Performance Liquid Chromatography (HPLC)
- Bridged ethylene hybrid (BEH)
- BEH 300A C4 column
ANSWER
Yes, but see additional comments for guidance.
ADDITIONAL INFORMATION
The presence of detergents such as SDS can alter the performance of a biomolecule separation on a BEH 300 C4 column. These detergents will not physically harm the column packing, but the chromatography will be negatively affected by these detergents. Routine monitoring with injections of a standard, as well as and column cleaning, will need to be performed regularly.
The best option is to perform sample cleanup prior to injection, to remove these detergents from the samples.
id108996, MSDS, UPBINARY