What is a good BEH SEC column choice to analyze a 100 kDa protein and aggregates up to octomers (800 kDa)? - WKB108383

ENVIRONMENT

• Ultra Performance Liquid Chromatography (UPLC)
• Ultra High Performance Liquid Chromatography (UHPLC)
• High Performance Liquid Chromatography (HPLC)
• ACQUITY columns
• Bridged ethylene hybrid (BEH)
• Size exclusion chromatography (SEC)
• Protein separation
• XBridge

ANSWER

For UPLC:
ACQUITY UPLC Protein BEH SEC, 450A, 2.5µm, 4.6mm ID columns for maximum sample throughput
XBridge Protein BEH SEC, 450A, 3.5µm, 7.8mm ID columns if ~50% lower sample throughput is OK

For HPLC:
XBridge Protein BEH SEC, 450A, 3.5µm, 7.8mm ID columns

ADDITIONAL INFORMATION

Also see: 

What is the molecular weight linear range for each of the BEH SEC columns?

BEH SEC Column Technology – Infographic

Protein SEC Columns

Guide to Size-Exclusion Chromatography (SEC) of mAb Aggregates, Monomers, and Fragments

ACQUITY UPLC BEH SEC Columns  and Standards Care & Use Manual

XBridge Protein BEH SEC Columns & Standards Care & Use Manual