What should I consider when I pick a Prep column for my HPLC? - WKB107692
Article number: 107692
ENVIRONMENT
- Preparative chromatography
- Prep column
ANSWER
Consider the following questions when selecting a Prep column for your LC system:
1) What is the current dimension of your analytical column and how much are you able to load onto that?
2) What is the flow rate used with the current analytical column?
3) Does the sample need to be heated to remain soluble?
4) What is the flow rate capability of the LC pump that you will be using for Prep chromatography (i.e., 1-10 mL/min, up to 100 mL/min)?
ADDITIONAL INFORMATION
For additional information, see How do I determine the flow rate scaling from one preparative column ID to another? and Does Waters have a calculator to help me scale from analytical to preparative chromatography?
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