How can I prevent the cysteine monomer from reacting with itself when derivatizing at high pH with any reagent under such conditions? - WKB98140
Article number: 98140
ENVIRONMENT
- cysteine
- amino acid
- derivatize
- AccQ-Tag
- PICO-Tag
- alkylation
- reaction
ANSWER
To prevent the reaction of cysteine to itself under high pH conditions, perform alkylation of the free sulfhydryl group on the cysteine.
ADDITIONAL INFORMATION
4-VP (4-vinylpyridine) is used to "cap" the free sulfhydryl group prior to derivatization with AccQ-Tag Ultra.
For additional information, see 2.3.2 Alkylation of cysteine (reduction followed by alkylation of cysteine) in the Comprehensive Guide to Hydrolysis and Analysis of Amino Acids
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