Can I react the free sulfhydryl group of glutathione with NEM and still derivatize the primary amine with AccQ•Tag reagent? - WKB84358
Article number: 84358
ENVIRONMENT
- AccQ•Tag
- AccQ-Fluor
- Glutathione
ANSWER
Yes. Un-reacted sulfhydryl group of glutathione can be protected with a reagent like NEM, and this NEM–amino acid conjugate can then be derivatized with AccQ•Tag reagent. The primary amine of glutathione will react with the AccQ•Tag reagent.
ADDITIONAL INFORMATION
NEM = N-EthylMaleimide
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