Where can I find a protocol to make mobile phase hexylammonium acetate for oligonucleotide applications, and why would one use it? - WKB84206

ENVIRONMENT

• UPLC
• Oligonucleotide applications
• Mobile phase
• Hexylammonium acetate (HAA)

ANSWER

Where: In this application note, you can find a description of the preparation of HAA:

Hexylammonium Acetate as an Ion-Pairing Agent for IP-RP LC Analysis of Oligonucleotides (720003361en)

(Scroll down to find the PDF of the application note.)

Why: The cost associated with the use of HAA is significantly lower than that of HFIP-based mobile phases. Moreover, the resolution of oligonucleotides is improved in non-denaturing for duplexes. 

ADDITIONAL INFORMATION