Will DMSO interfere with the AccQ•Tag Ultra derivatization? - WKB55631
Article number: 55631
ENVIRONMENT
- Amino acid analysis
- Derivatization reaction
- AccQ•Tag Ultra
- Dimethylsulfoxide (DMSO)
ANSWER
No, provided that the following conditions apply:
- The derivatization cocktail is buffered properly
- The DMSO has not extracted amine-containing components from storage that could be derivatized
- There is sufficient primary and secondary to AccQ•Tag Ultra reagent ratio to ensure complete derivatization
- The amount of DMSO in the sample does not boost the amount of overall organic in the derivatization cocktail above 20% of the total volume
ADDITIONAL INFORMATION
For additional information, see What should I do to prevent chromatographic contamination in the amino acid kits like Accq-Tag and Pico-Tag?
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