Can I use RapiGest SF to prevent protein binding without denaturing a protein? - WKB231856
Article number: 231856
ENVIRONMENT
- Protein digestion
- RapiGest SF
ANSWER
Possibly, but RapiGest SF was not designed for this use.
ADDITIONAL INFORMATION
Due to the complex nature of proteins, experimentation would be needed to determine the concentration at which RapiGest SF could inhibit protein binding without denaturing each protein.
Detergents such as Tween or Triton X may be screened for this purpose.
Use of low ionic strength buffers may also be screened for this purpose.
RapiGest SF is designed to denature proteins in order to allow trypsin (or other enzymes) better access to cleavage sites during a protein digestion.
RapiGest SF Surfactant Care & Use Manual
RapiGest SF Surfactant: An Enabling Tool for In-Solution Enzymatic Protein Digestions
Significantly Improved In-Solution Tryptic Digestion of Proteins (Poster)
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