Why does one GlycoWorks RapiFluor-MS protocol call for a 3% RapiGest SF concentration while a different protocol calls for a 5% RapiGest SF? - WKB194900
Article number: 194900
ENVIRONMENT
- N-released glycan analysis
- Solid-phase extraction (SPE)
- Buffer compatibility
- GlycoWorks RapiFluor-MS
ANSWER
The protocol that uses a 3% RapiGest SF concentration calls for addition of 10 µL of the RapiGest SF solution to the glycoprotein.
This protocol is designed for a QC lab environment; the addition (10 µL) helps minimize any error caused by pipettes.
ADDITIONAL INFORMATION
Results obtained from using both protocols are comparable.
The protocol that calls for 3% RapiGest SF solution and the use of 10 µL of the RapiGest solution is in these documents:
- GlycoWorks RapiFluor-MS Quick Start Protocol - 24 Sample
- GlycoWorks RapiFluor-MS Quick Start Protocol - 96 Sample
The protocol that calls for 5% RapiGest SF solution and the use of 6 µL of the RapiGest SF solution is in these documents:
- GlycoWorks RapiFluor-MS N-Glycan Kit - 24 Samples Care and Use Manual
- GlycoWorks RapiFluor-MS N-Glycan Kit – 96 Sample - Care and Use Manual
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