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Using the GlycoWorks RapiFluor-MS protocol, is it safe to use more Rapid PNGaseF to digest samples that contain more than 1.5 mg/mL protein? - WKB194896

Article number: 194896

ENVIRONMENT

  • N-released glycan analysis
  • Solid-phase extraction (SPE)
  • Buffer compatibility
  • GlycoWorks RapiFluor-MS

ANSWER

Yes

ADDITIONAL INFORMATION

You can dilute the concentrated sample to 1.5 - 2 mg/mL for reaction instead of using extra PNGase F.

However, adding more PNGase F will not impact labeling efficiency and should not cause any side effects.
 

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