Using the GlycoWorks RapiFluor-MS protocol, is it safe to use more Rapid PNGaseF to digest samples that contain more than 1.5 mg/mL protein? - WKB194896
Article number: 194896
ENVIRONMENT
- N-released glycan analysis
- Solid-phase extraction (SPE)
- Buffer compatibility
- GlycoWorks RapiFluor-MS
ANSWER
Yes
ADDITIONAL INFORMATION
You can dilute the concentrated sample to 1.5 - 2 mg/mL for reaction instead of using extra PNGase F.
However, adding more PNGase F will not impact labeling efficiency and should not cause any side effects.
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