How often should I change my TEA-HFIP mobile phase when performing ion-pair reverse-phase separations of oligonucleotides? - WKB193681
ENVIRONMENT
- Hexafluoroisopropanol (HFIP)
- Triethylamine (TEA)
- Ion-pair reverse-phase (IP-RP)
- Oligonucleotides
- Oligonucleotide separation technology (OST)
ANSWER
Every 24 hours for LC/MS. No more than 5 days for UV analysis.
ADDITIONAL INFORMATION
Ensure that the mobile phase bottles are well sealed.
MS sensitivity can decline after only 24 hours when using any amine-HFIP buffer system.
For additional information see Best Practices for Oligonucleotide analysis using Ion Pair Reverse Phase Liquid Chromatography: columns and chemistries
Oligonucleotide Separation Technology Columns Web Page
Oligonucleotide Separation Technology (OST) Columns Brochure
ACQUITY UPLC and ACQUITY PREMIER Oligonucleotide C18 Columns Care & Use Manual
XBridge Oligonucleotide C18 Columns Care & Use Manual
Oligonucleotide Applications Notebook
Ion-Pairing Systems for Reversed-Phase Chromatography Separation of Oligonucleotides (Poster)
ACQUITY UPLC OST C18 Method Guidelines
XBridge OST C18 Method Guidelines
id193681, eluent