Is there a way to completely remove intact RapiGest SF from a pH 7 sample, after protein denaturation? - WKB123443

ENVIRONMENT

• RapiGest SF
• Solubilization Factor (SF)

ANSWER

There are two ways to remove RapiGest SF:

1. Acid degradation under low pH
2. Molecular weight cutoff filter or gel filtration tips

ADDITIONAL INFORMATION

1. Acid degradation under low pH:
   • The pellet is visible when protein is very diluted.
   • Even if there is a pellet, it is hard to see a tiny colorless pellet on a transparent Eppendorf tube.
2. MW cutoff spin column or gel filtration tips:
   • To remove low-MW species.
   • Works for RapiGest SF removal.
   • Other scientists have used gel filtration tips to remove low-MW species.