TP385 Design and performance of a second generation cyclic ion mobility-enabled Q-ToF
KEVIN GILES, Jakub Ujma, Jason Wildgoose, Martin R. Green, Keith Richardson, David Langridge, Nick Tomczyk
Waters Corporation, Stamford Avenue, Altrincham Road, Wilmslow, Cheshire, UK
Introduction
Interest in ion mobility (IM) separation continues to grow and it is now utilised over a broad range of application areas. Over the last five years or so there has been a notable advance in ion mobility technology coupled with mass spectrometry with significant increases in mobility resolution and the promise of additional instrument functionality. One such system has been a T-Wave driven multi-pass cyclic ion mobility separator embedded in a Q-ToF instrument. Here we report on the preliminary design and performance of a second generation research platform with enhancements to the ion optics, mass analyser, detection system and instrument control.
Methods
The second generation research platform comprises a modified Synapt G2-S Q-IM-ToF instrument. The new cyclic IM device replaces the standard mobility cell and has modified entry and exit ion guides. Ion transport from the IM device to the ToF consists of a new beam conditioning ion guide; furthermore the ToF geometry has been modified to provide increased mass resolution. The detection system has a new analogue-to-digital converter (ADC) with increased dynamic range and the prospect of increased mobility record length. The instrument control software comprises a more adaptable interface to facilitate more rapid development of the platform and its modes of operation.
Preliminary Data or Plenary Speakers Abstract
The new cyclic IM cell is constructed of quadrants as opposed to the original single element design which makes it easier to construct. Initial evaluation indicates that this new design performs as well as the previous version, with mobility resolution in excess of 200 being realised. The ion entry and exit guides to the cyclic IM have been modified to facilitate a broader m/z transmission window at the higher mobility cell pressure. Modifications to both the instrument control software and firmware for the cyclic IM are in progress that will enable multi-functional capability such as IMSn. The ion transport from the cyclic IM comprises a quadrupole ion guide-based collision cell with axial field which both maintains the mobility separation and conditions the ion beam for high transmission into the ToF analyser. The ToF itself is of increased length compared to a standard instrument and m/z resolution around 120k has been obtained. The new ADC has a dual gain architecture providing up to 60x increase in dynamic range over the standard instrument and work is underway to provide increased mobility record length to account for the longer separation times in the cyclic mobility device.
Novel Aspect
A high performance, flexible cyclic ion mobility-enabled Q-ToF instrument