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Waters

2777 Autosampler: Low sensitivity with the detector or no peaks detected - WKB93187

Article number: 93187

SYMPTOMS

  • Low sensitivity or no peak detected.
  • When run with different samples, there are still no peaks and all samples give the same chromatogram.
  • There is no error on the detector side.
  • The mobile phase can be delivered normally.
  • Replacing the column does not resolve the issue
  • The syringe is drawing the sample normally and the injections are also being performed.

ENVIRONMENT

  • 2777 Sample Manager
  • ACQUITY UPLC BSM
  • ACQUITY UPLC Column Manager
  • ACQUITY UPLC PDA Detector
  • ACQUITY UPLC ELS Detector
  • TQ Detector

CAUSE

Clogging or contamination within the injection valve pod.

Clogged or dirty needle seal.

FIX or WORKAROUND

  1. Remove the needle guide for port 2 of the injection valve pod.
  2. Remove the needle seal (700002208) inside.
  3. If any clogging or contamination is presents, remove it. If it cannot be removed, replace the parts with a new one.
  4. Remove the flow path connection on the injection valve pod.
  5. Deliver methanol from BSM and connect it to each port for reverse flushing or clogging removing.
  6. If the clogging persists, replace the inject valve pod (700011083)

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