How to perform the ACQUITY UPLC H-Class needle seal readiness test - WKB99826

OBJECTIVE or GOAL

• Understand the procedure for performing the ACQUITY UPLC H-Class Needle Seal Preparation Test
• In the event of poor FTN reproducibility, check for needle seal leaks if the syringe leak test is normal.

ENVIRONMENT

• ACQUITY UPLC H-Class
• ACQUITY UPLC I-Class
• Sample Manager - Flow Through Needle PLUS (SM-FTN PLUS)

PROCEDURE

1. Set a suitable flow rate ~0.6ml/min (low flow rate can cause a fail even if everything is fine) on the solvent manager to obtain a column backpressure of between 6000-8000 psi.
2. Perform the Needle Seal Readiness test, you can find this in the ACQUITY console > SMFTN > Maintain > Needle Seal Readiness Test.
   
3. Click Start.
   
4. Review results
   

ADDITIONAL INFORMATION

See the video Needle seal readiness test.

150mm columns may cause the test to flag a false fail.