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Waters

Poor reproducibility on H-Class SM-FTN - WKB93273

Article number: 93273

SYMPTOMS

  • High %RSD on a customer method.
  • All system diagnostic tests pass, including Needle Seal Readiness test.

     

ENVIRONMENT

  • ACQUITY UPLC H-Class
  • ACQUITY UPLC H-Class Bio
  • ACQUITY UPLC H-Class PLUS
  • ACQUITY UPLC H-Class PLUS Bio

CAUSE

Leak around needle and needle seat causing high %RSD.

FIX or WORKAROUND

  1. Replace needle, needle seat, and needle guide.
  2. Part numbers (Needle, 15uL SS w/Guide and Seat)
    • H-Class-700005215
    • H-Class Bio-700005421
    • H-Class Plus-700011628
    • H-Class Bio Plus-700011658
  3. Characterize the needle z axis.
  4. Characterize the needle seal.
  5. Perform the Needle Seal Readiness test.

     

ADDITIONAL INFORMATION

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