UV/Vis Absorbance Detector - Lower response than expected - WKB89944
Article number: 89944
SYMPTOMS
- Lower-than-expected peak responses when compared to other similar systems.
- Retention times are ok.
- Peak Areas reproducible.
- Peak Heights reproducible.
ENVIRONMENT
- 2487 Dual-Wavelength Absorbance Detector
- 2489 UV/Vis Detector
- 2996 PDA Detector
- 2998 Photodiode Array Detector
- ACQUITY UPLC Tunable UV Detector
- ACQUITY UPLC PDA Detector
- ACQUITY UPLC PDA eλ Detector
CAUSE
The detector has not been powered-off for a significant length of time, so the calibration against the internal filters has drifted over time. When you select a wavelength for a method, the detector is actually reporting back from the side of a calibration peak rather than the top.
FIX or WORKAROUND
Power cycle the Detector and allow the Detector to reinitialize and perform internal self-adjustments.
ADDITIONAL INFORMATION