- H-Class SM-FTN
- I-Class SM-FTN
- UPLC ACQUITY I-Class
The volume of needle wash consumed should be about 1.0 to 2.5 mL.
The amount of needle wash consumed is relative to the programmed time and the viscosity of the needle wash. The answer assumes that the instrument is programmed using the default post-injection needle wash of 6 seconds and that the chosen needle wash is 90% water, 10% acetonitrile.
For information on the flow rate for the needle wash, refer to article 18339.