NanoACQUITY or M-Class System overpressure - WKB8168

SYMPTOMS

• H/W overpressure error for the nano Binary Solvent Manager (nBSM).
• Start flow and it quickly spikes to a high pressure.

ENVIRONMENT

• NanoACQUITY
• ACQUITY M-Class

CAUSE

Debris blocking the lines.

FIX or WORKAROUND

1. Flush with high organic in Analytical mode overnight at as high a flow rate as possible without getting overpressure.
2. If there is still high pressure, disconnect the trap column from port 6 of the inject valve and plumb some PEEK tubing to port 6 going to waste (or place a wad of Kim wipes below the inject valve to catch the solvent).  
3. Prime A and B, and then flush with acetonitrile/water/methanol/isopropyl alcohol and 0.2% formic acid for at least three hours at 15 uL/min. 
4. Prime and flush with fresh normal A and B solvents (water for A and acetonitrile for B) for 15 minutes at 15 uL/min. 
5. Stop flow and attach the trap column. 

ADDITIONAL INFORMATION