What solvent should be in the flow cell when calibrating the 2475? - WKB81404
Article number: 81404
ENVIRONMENT
- 2475 Multi-Wavelength Fluorescence Detector
- ACQUITY UPLC FLR Detector
ANSWER
It is suggested that water be in the flow cell when performing the manual calibration.
ADDITIONAL INFORMATION
Other clear solvents can be used, but you do not want to use anything with a high background that could interfere with the recognition of the erbium peaks. If water is running through the cell, then wavelength calibration, PMT calibration, and normalization can be run in succession without changing mobile phase.
id81404, 2475, 2475B, A-10FL, A-30FL, eluent, photomultplier tube, UPFLRARC, UPFLRDET, UPPFLR