Different chromatography on different FLRs - WKB64135

SYMPTOMS

• Response for assay varies across different FLR systems in the lab
• Passes Raman signal to noise and calibration
• Gain linearity varies across different FLR systems

ENVIRONMENT

• ACQUITY UPLC FLR Detector

CAUSE

Systems are not normalized to the same level.

FIX or WORKAROUND

1. Run 100% water (LCMS-grade if possible) through the FLR at 0.5 mL/min for an hour with lamp on.
2. Normalize FLR.

ADDITIONAL INFORMATION

Use the highest-quality water for calibration, normalization, and Raman signal to noise.