ACQUITY UPLC SM-FTN peaks elute late - WKB59196

SYMPTOMS

• Peaks elute later than expected
• Sample manager passes all diagnostics
• Solvent manager passes dynamic leak test to vent valve
• Solvent manager passes dynamic leak test to column WITHOUT including needle or seal
• Solvent manager fails dynamic leak test to column including needle and seal

ENVIRONMENT

• ACQUITY UPLC H-Class SM-FTN
• ACQUITY UPLC H-Class
• ACQUITY UPLC I-Class
• ACQUITY UPLC I-Class SM-FTN

CAUSE

Leak in SM-FTN between needle and vespel seal

FIX or WORKAROUND

Replace the needle using needle seal (700005215/Plus 700011628) and needle seat port (700005234).

Perform dynamic leak test up to column, including needle and seal

ADDITIONAL INFORMATION

Late eluting peaks are the result of incorrect mobile phase composition.