- Retention time initially shifts to a longer retention time than expected after refreshing solvents and repriming the pump.
- Retention time gradually reverts back to expected retention time after several days and then remains constant until the solvents are refreshed again.
- ACQUITY UPLC H-Class
Air within pump
FIX or WORKAROUND
Prime all lines with IPA or methanol for 3 minutes, and then reprime with mobile phases.