- Needle seal readiness test fails
- Low system pressure
- Pressure fluctuation after injection
- Inaccurate flow rate, lower volume delivered than expected
- ACQUITY UPLC H-Class
- ACQUITY UPLC I-Class
System pressure did not stabilize before test or was too low.
A leak is present on the system causing a high pressure difference value.
FIX or WORKAROUND
Set flow should be similar to initial testing (0.6ml/min) with similar pressure (~6000 - 8000psi) and allow pressure to stabilize before running this test.
If the test continues to fail replace injector parts (Seal, needle and seat port) and re-run the test.
Seat, Vespel w anti-rot (H-Class/I-Class), 405011492
Assy, Needle 15uL w Guide and Seat (H-Class), 700005215
Seat Port Tubing 0.004 ID (H-Class), 700005234
Sample Needle Kit, 15uL (I-Class), 700008977
Seat Port Assembly, .003 ID (I-Class), 700006056
Delta, pressure ripple