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Needle seal readiness test fails on ACQUITY H-Class/I-Class - WKB5500

Article number: 5500

SYMPTOMS

  • Needle seal readiness test fails
  • Low system pressure
  • Pressure fluctuation after injection
  • Inaccurate flow rate, lower volume delivered than expected

ENVIRONMENT

  • ACQUITY UPLC H-Class
  • ACQUITY UPLC I-Class
  • SM-FTN

CAUSE

System pressure did not stabilize before test or was too low.

A leak is present on the system causing a high pressure difference value.

Insufficient priming.

FIX or WORKAROUND

Set flow should be similar to initial testing (0.6ml/min) with similar pressure (~6000 - 8000psi) and  allow pressure to stabilize before running this test.

If the test continues to fail replace injector parts (Seal, needle and seat port) and re-run the test.

ADDITIONAL INFORMATION

Seat, Vespel w anti-rot (H-Class/I-Class), 405011492

Assy, Needle 15uL w Guide and Seat (H-Class), 700005215

Seat Port Tubing 0.004 ID (H-Class), 700005234

Kit, Textured Needle FTN-15µL SS w/guide and seat (H-Class Plus), 700011628

Sample Needle Kit, 15uL (I-Class), 700008977

Seat Port Assembly, .003 ID (I-Class), 700006056

 

Delta, pressure ripple

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